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I-Dengue Virus Typing Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
Intshayelelo
Le khithi yenzelwe ukufumanisa ukuchwetheza okusemgangathweni kwe-dengue virus nucleic acid kwi-serum yabantu okanye iisampulu zeplasma.Le khithi isekelwe kwisiqwenga esikhethekileyo kuyo yonke i-genome yohlobo lwe-dengue virus uhlobo 1~4 ukuyila iiprimers ezithile kunye ne-TaqMan fluorescent probes kudidi ngalunye, kwaye iqonde ukubhaqwa okukhawulezileyo kunye nokuchwetheza intsholongwane ye-dengue nge-PCR yexesha lokwenyani le-fluorescent.
Iiparamitha
* Uhlobo lomfanekiso: iSerum okanye iPlasma * Izixhobo zesicelo: I-ABI 7500 Real-Time PCR System;Bio-Rad CFX96;Roche LightCycler480;Inkqubo ye-PCR ye-SLAN. * Ukugcinwa -25℃ ukuya kwi-8℃ engavulwanga kwaye ikhusele ekukhanyeni kwiinyanga ezili-18Amacandelo 48T/Kit Izithako eziphambili DENV-Uhlobo reaction umxube, lyophilized 2 iityhubhu Iiprimers, probes, PCR reaction buffer, dNTPs, Enzyme, njl. DENV ulawulo olulungileyo, lyophilized 1 ityhubhu I-Plasmids ye-tandem ye-dengue virus yohlobo lwe-1-4 yokufumanisa amaqhekeza ekujoliswe kuyo Ulawulo olubi (Amanzi acocekileyo) 1.5mL Amanzi ahlambulukileyo Incwadi yokusebenzisa Iyunithi e-1 / Ukusebenza
•NgokuKhawuleza: Elona xesha lifutshane lokukhulisa iPCR phakathi kwemveliso efanayo.•Ubuntununtunu obuphezulu kunye nobungcali: Ikhuthaza ukufunyaniswa kwangoko kunyango olukhawulezileyo.•Isakhono esibanzi sokuchasana nokuphazamiseka.Amanyathelo okusebenza
