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ULungele-ukuSebenzisa uMxube oyi-PCR Master
Intshayelelo
Read-to-Use PCR Master Mix ilungele ukusetyenziswa, lyophilized master mix for high-effective high-effective PCR realtime amplification enokuthi isetyenziswe ukubhaqwa ngokukhawuleza kweesampulu zeDNA. Umxube uquka kabini-ivaliwe eshushu-isiqalo Super HP Taq I-DNA polymerase, i-MgCl2 kunye ne-dNTPs.Yenza ngokulula umxube oyintloko ngokudibanisa amanzi ebakala lePCR kunye nethempleyithi yakho, iTaqman probes kunye neeprimers kumthamo opheleleyo wama-20 µl.
Iiparamitha
* Gcina ku -25 ℃ ~ 8 ℃.Ugcino olomileyo olutywiniweyo, olungenabumanzi. * Igama langaphambili lale khithi yiRealtime PCR Master Mix (ene-dNTPs, i-lyophilized).Inombolo yeCAT. Icandelo Inkcazo Ubungakanani Phawula KY133-01 Ilula kakhulu iSYBR eluhlaza qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48Iityhubhu 8-umcu wequla, 0.1mL PCR-grade amanzi 1.5mL/Iityhubhu 1 Umbhobho Cryotube, 2.0 mL KY133-02 Ilula kakhulu iSYBR eluhlaza qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48Iityhubhu 8-umcu wequla, 0.2mL PCR-grade amanzi 1.5mL/Iityhubhu 1 Umbhobho Cryotube, 2.0 mL KY133-03 Ilula kakhulu iSYBR eluhlaza qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi Imibhobho emi-2 Cryotube, 2.0 mL PCR-grade amanzi 1.5mL/Iityhubhu 1 Umbhobho Cryotube, 2.0 mL KY133-04 Ilula kakhulu iSYBR eluhlaza qPCR Master Mix (ene-dNTPs, i-lyophilized) 500T/ikhithi 1Tube / PCR-grade amanzi 10mL 1Ibhotile / Ukusebenza
• Usetyenziso olubanzi: Iyasebenza ekubhaqweni okuninzi.• Uvakalelo oluphezulu: Ukusebenza okugqwesileyo kugxininiso lweetemplates eziphantsi.• Uncedo: Pre-mixed kwaye simahla ukusetyenziswa. -
Super-Easy SYBR Green RT-qPCR Master Mix
Intshayelelo
Super lula SYBR Green RT-qPCR Master Mix(ene dNTPs, lyophilized) ilungele ukusetyenziswa, lyophilizedmastermixfor high-efficiency RT-qPCR amplification enokusetyenziswa ukubhaqa ngokukhawuleza DNA okanye RNAsamples.I-Themixin iquka i-double-blocked hot-start-start Super HP Taq DNA polymerase, M-MLV reverse transcriptase (RNaseH-), MgCl2, SYBR Idayi eluhlaza kunye ne-dNTPs.Yenza ngokulula umxube oyintloko ngokudibanisa amanzi ebakala le-PCR kunye nethempleyithi yakho, iTaqman probes kunye neeprimers kumthamo opheleleyo wama-20 µl.
Iiparamitha
* Gcina ku -25 ℃ ~ 8 ℃.Ugcino olomileyo olutywiniweyo, olungenabumanzi. *Le khithi igama langaphambili yi-Super-Easy SYBR Green RT-qPCR Master Mix (ene-dNTP, lyophilized). UkusebenzaInombolo yeCAT. Icandelo Inkcazo Ubungakanani Phawula KY135-01 Ilula kakhulu iSYBR eluhlaza RT-qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48Iityhubhu 8-umcu wequla, 0.1mL PCR-grade amanzi 1.5mL/Tube 1Tube Cryotube, 2.0 mL KY135-02 Ilula kakhulu iSYBR eluhlaza RT-qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48Iityhubhu 8-umcu wequla, 0.2mL PCR-grade amanzi 1.5mL/Tube 1Tube Cryotube, 2.0 mL KY135-03 Ilula kakhulu iSYBR eluhlaza RT-qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi Imibhobho emi-2 Cryotube, 2.0 mL PCR-grade amanzi 1.5mL/Tube 1Tube Cryotube, 2.0 mL KY135-04 Ilula kakhulu iSYBR eluhlaza RT-qPCR Master Mix (ene-dNTPs, i-lyophilized) 500T/ikhithi 1Tube / PCR-grade amanzi 10mL 1Ibhotile / • Ukuchaneka: Umngcipheko ophantsi wosulelo• Uvakalelo oluphezulu: Ukusebenza okugqwesileyo kugxininiso lweetemplates eziphantsi.• Uncedo: Pre-mixed kwaye simahla ukusetyenziswa. -
Ilula kakhulu SYBR Green qPCR Master Mix
Intshayelelo
Ilula kakhulu iSYBR Green qPCR Master Mix (ene-dNTPs,lyophilized) ilungele ukusetyenziswa, i-lyophilized master mixforhigh-efficiency yenyani yokukhulisa i-PCR enokusetyenziselwa ukubonwa ngokukhawuleza kweesampulu ze-DNA.Umxube ubandakanya i-super HP Taq DNA polymerase evaliwe kabini, i-PCR Buffer, i-MgCl2, i-SYBR eluhlaza kunye ne-dNTPs.Yenza ngokulula umxube oyintloko ngokudibanisa amanzi e-PCR-grade kunye netemplate yakho kunye ne-primers kumthamo opheleleyo we-20µl. Ekubeni iimveliso ze-PCR eziveliswa yi-lyophilized PCR Master Mix ziqulethe i-3'-A ii-overhangs, zinokuthi zifakwe ngqo kwi-T-vectors.Iimveliso ze-RT-qPCR zifanelekile ulandelelwano.
Iiparamitha
* Gcina ku -25 ℃ ~ 8 ℃.Ugcino olomileyo olutywiniweyo, olungenabumanzi. *Igama langaphambili lale khithi lilula kakhulu iSYBR Green qPCR Master Mix (ene-dNTPs, i-lyophilized)Inombolo yeCAT. Icandelo Inkcazo Ubungakanani Phawula KY136-01 Ilula kakhulu iSYBR eluhlaza qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48Iityhubhu 8-umcu wequla, 0.1mL PCR-grade amanzi 1.5mL/Iityhubhu 1 Umbhobho Cryotube, 2.0 mL KY136-02 Ilula kakhulu iSYBR eluhlaza qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48Iityhubhu 8-umcu wequla, 0.2mL PCR-grade amanzi 1.5mL/Iityhubhu 1 Umbhobho Cryotube, 2.0 mL KY136-03 Ilula kakhulu iSYBR eluhlaza qPCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi Imibhobho emi-2 Cryotube, 2.0 mL PCR-grade amanzi 1.5mL/Iityhubhu 1 Umbhobho Cryotube, 2.0 mL KY136-04 Ilula kakhulu iSYBR eluhlaza qPCR Master Mix (ene-dNTPs, i-lyophilized) 500T/ikhithi 1Tube / PCR-grade amanzi 10mL 1Ibhotile / Ukusebenza
• Usetyenziso olubanzi: Iyasebenza ekubhaqweni okuninzi.• Uvakalelo oluphezulu: Ukusebenza okugqwesileyo kugxininiso lweetemplates eziphantsi.• Uncedo: Pre-mixed kwaye simahla ukusetyenziswa. -
Inyathelo elinye RT-PCR Master Mix
Intshayelelo
Inyathelo elinye le-RT-PCR Master Mix ilungele ukusetyenziswa, i-lyophilized master mix kwi-high-efficiency RT-qPCR yokukhulisa enokuthi isetyenziswe ukubhaqa ngokukhawuleza kwe-DNA okanye iisampulu ze-RNA.Umxube uquka i-double-blocked hot-start Super HP Taq DNA polymerase, M-MLV reverse transcriptase (RNaseH-), MgCl2 anddNTPs.Yenza ngokulula umxube obalaseleyo ngokudibanisa amanzi odidi lwePCR kunye netemplate yakho, iTaqman iphanda kwaye iqale ibe ngumthamo opheleleyo wama-20 µl.
Iiparamitha
* Gcina ku -25 ℃ ~ 8 ℃.Ugcino olomileyo olutywiniweyo, olungenabumanzi. *Le khithi igama langaphambili linyathelo elinye RT-qPCR Master Mix (kunye dNTPs, lyophilized).Inombolo yeCAT. Icandelo Inkcazo Ubungakanani Phawula KY132-01 Inyathelo elinye le-RT-PCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48Iityhubhu 8-umcu wequla, 0.1mL PCR-grade amanzi 1.5mL/Tube 1Tube Cryotube, 2.0mL KY132-02 Inyathelo elinye le-RT-PCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48Iityhubhu 8-umcu wequla, 0.2mL PCR-grade amanzi 1.5mL/Tube 1Tube Cryotube, 2.0mL KY132-03 Inyathelo elinye le-RT-PCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi Imibhobho emi-2 Cryotube, 2.0mL PCR-grade amanzi 1.5mL/Tube 1Tube Cryotube, 2.0mL KY132-04 Inyathelo elinye le-RT-PCR Master Mix (ene-dNTPs, i-lyophilized) 500T/ikhithi 1Tube / PCR-grade amanzi 10mL/Tube 1Tube / Ukusebenza
• Ukuchaneka: Umngcipheko ophantsi wosulelo• Uvakalelo oluphezulu: Ukusebenza okugqwesileyo kugxininiso lweetemplates eziphantsi.• Uncedo: Pre-mixed kwaye simahla ukusetyenziswa. -
Impahla engekasilwa
Ukuhlangabezana neemfuno zemarike ezibanzi, Sibonelela ngeenkonzo zemveliso yemathiriyeli ekrwada yeenkqubo ezichanekileyo zokumelana ne-enzymatic eziphucula ukuchaneka kwePCR.Sinelunda ukunazisa iinkqubo zethu ezintandathu zee-enzyme.
