IKit yoVavanyo lwe-Nucleic Acid yeMP (indlela yePCR-fluorescence probe)
Inkcazelo emfutshane:
Intshayelelo
IMycoplasma pneumoniae iqala kancinane, ibe neempawu ezinjengomqala obuhlungu, intloko ebuhlungu, ifiva, ukudinwa, ukuqaqamba kwezihlunu, ukungakuthandi ukutya, isicaphucaphu nokugabha ekuqaleni kwesi sigulo.Ukuqala komkhuhlane ngokuqhelekileyo kuphakathi, kwaye iimpawu zokuphefumla ziyabonakala emva kweentsuku ezi-2-3, zibonakaliswa yi-paroxysmal ukhohlokhohlo olucaphukisayo, ngakumbi ebusuku, kunye nenani elincinci le-mucous okanye i-sputum ye-mucopurulent, ngamanye amaxesha ngegazi kwi-sputum, kunye ne-dyspnea. kunye neentlungu zesifuba.Abantu badla ngokuba sesichengeni seMycoplasma pneumoniae, ingakumbi kubantwana abasaqala ukuya esikolweni, abakubudala bokuhamba isikolo nabakwishumi elivisayo.
Le khithi yenzelwe ukufumanisa ukuchwetheza okusemgangathweni kweMycoplasma pneumoniae nucleic acid kwiserum yabantu okanye iisampulu zeplasma.Le khithi isebenzisa ijini ye-p1 yolandelelwano olugcinwe kakhulu kwi-Mycoplasma pneumoniae gene njengommandla ekujoliswe kuwo, kwaye iyila iiprimers ezithile kunye ne-TaqMan fluorescent probes kwaye iqonde ukubhaqwa okukhawulezileyo kunye nokuchwetheza intsholongwane yedengue nge-PCR yexesha lokwenyani le-fluorescent.
Iiparamitha
| Amacandelo | 48T/Kit | Izithako eziphambili |
| MP/IC reaction umxube, lyophilized | 2 iityhubhu | Iiprimers, probes, PCR reaction buffer, dNTPs, Enzyme, njl. |
| MP ulawulo olulungileyo, lyophilized | ityhubhu e-1 | Amasuntswana e-pseudoviral abandakanya ulandelelwano olujoliswe kuyo kunye nolandelelwano lolawulo lwangaphakathi |
| Ulawulo olubi (Amanzi acocekileyo) | 3mL | Amanzi ahlambulukileyo |
| DNA ulawulo lwangaphakathi, lyophilized | ityhubhu e-1 | Amasuntswana e-pseudoviral aquka i-M13 |
| IFU | Iyunithi e-1 | Incwadi yoMyalelo woMsebenzisi |
Ukusebenza
Amanyathelo okusebenza
MP Nucleic Acid Test Kit(Indlela yePCR-fluorescence probe)
Intshayelelo
Mycoplasma pneumoniaeiqala kancinane, ibe neempawu ezinjengomqala obuhlungu, intloko ebuhlungu, ifiva, ukudinwa, ukuqaqamba kwezihlunu, ukungakuthandi ukutya, isicaphucaphu nokugabha ekuqaleni kwesi sigulo.Ukuqala komkhuhlane ngokuqhelekileyo kuphakathi, kwaye iimpawu zokuphefumla ziyabonakala emva kweentsuku ezi-2-3, zibonakaliswa yi-paroxysmal ukhohlokhohlo olucaphukisayo, ngakumbi ebusuku, kunye nenani elincinci le-mucous okanye i-sputum ye-mucopurulent, ngamanye amaxesha ngegazi kwi-sputum, kunye ne-dyspnea. kunye neentlungu zesifuba.Abantu badla ngokuba sesichengeni seMycoplasma pneumoniae, ingakumbi kubantwana abasaqala ukuya esikolweni, abakubudala bokuhamba isikolo nabakwishumi elivisayo.
Le khithi yenzelwe ukufumanisa ukuchwetheza okusemgangathweni kweMycoplasma pneumoniae nucleic acid kwiserum yabantu okanye iisampulu zeplasma.Le khithi isebenzisa ijini ye-p1 yolandelelwano olugcinwe kakhulu kwi-Mycoplasma pneumoniae gene njengommandla ekujoliswe kuwo, kwaye iyila iiprimers ezithile kunye ne-TaqMan fluorescent probes kwaye iqonde ukubhaqwa okukhawulezileyo kunye nokuchwetheza intsholongwane yedengue nge-PCR yexesha lokwenyani le-fluorescent.
Ukusebenza
Amanyathelo okusebenza
| Amacandelo | 48T/Kit | Izithako eziphambili |
| MP/IC reaction umxube, lyophilized | 2 iityhubhu | Iiprimers, probes, PCR reaction buffer, dNTPs, Enzyme, njl. |
| MP ulawulo olulungileyo, lyophilized | ityhubhu e-1 | Amasuntswana e-pseudoviral abandakanya ulandelelwano olujoliswe kuyo kunye nolandelelwano lolawulo lwangaphakathi |
| Ulawulo olubi (Amanzi acocekileyo) | 3mL | Amanzi ahlambulukileyo |
| DNA ulawulo lwangaphakathi, lyophilized | ityhubhu e-1 | Amasuntswana e-pseudoviral aquka i-M13 |
| IFU | Iyunithi e-1 | Incwadi yoMyalelo woMsebenzisi |
- Iprofayili yeMveliso yeHecin
